/ iPSC

ABX-006 hTSC 2003-derived iPSC Clone C6

Induced Pluripotent Stem Cell-derived from human Trophoblast Stem Cell

Overview

Accelerated Biosciences (AB)  human induced Pluripotent Stem Cells (iPSC) Clone C6 were derived from human trophoblast stem cells (hTSC) procured under current Good Tissue Practice (cGTP) and conform to Global Ethical Standards and Clinical Cell Regulations. All AB iPSC cells are derived from hTSC using a mRNA method, without Yamanaka factors.  
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All AB iPSC cells are manufactured by AB’s CDMO partner based on AB’s standard operating procedures.  ABX-006 cells are free of pathogens such as mycoplasma, bacteria, yeast, and fungus, and are guaranteed to achieve > 70% viability post-thaw at the indicated cell number.  ABX-006 cells also were characterized for karyotype, pluripotency, and Short Tandem Repeat (STR) cell line identity.  ABX-006 cells are intended for Research Use Only (RUO) and shall not be used for clinical or diagnostic purposes. Corresponding GMP cells are subject to commercial license.

Additional Info
  • Manufactured in USA
  • Research use only
  • Inquire with us for a commercial license
Pricing

$1500/vial of 1M cells in standard bank vials

(not including shipping and handling costs)

Contact for Certificate of Analysis
Contact Us to purchase ABX-006
Specifications 
Quality Control Testing
Culture Recommendations
Format :

1M cells/vial

Preparation :

2D Adherent Culture

Passage Number :

9

Storage :

Vapor Phase LN2

Karyotype:

46, XX

Pluripotency Markers :

OCT4, SSEA-4, NANOG, SOX2

iPSC Manufactured by :

Pluristyx, Inc. on behalf of Accelerated Biosciences Corp.

iPSC Expansion Location :

USA

Seed Stock Cell Line :

hTSC 2003

Seed Stock Source Location :

Taiwan, by Accelerated Biosciences Corp.

Test Description
Specification
Pass/Fail
Cell Viability Post-Thaw

≥70% viable cells

Pass

Viable Cell Number at Freeze

≥1x106 viable cells

Pass

Sterility

Negative

Pass

Mycoplasma

Negative

Pass

Karyotype Analysis

46, XX

Pass

Stem Cell/Pluripotency Marker Expression

≥70% OCT4+, SOX2+, SSEA-4+ triple positive cells

Pass

Identity (STR profiling)

100% match with reference

Pass

IDEXX h-IMPACT 1 Human Pathogens
Testing on Starting hTSC material.

Corynebacterium bovis
Corynebacterium sp. (HAC2)
EBV
HAdV
Hantaan
HCMV
Hepatitis A
Hepatitis B
Hepatitis C
HHV 6
HHV 8
HIV 1
HIV 2
HPV 16
HPV 18
HSV 1
HSV 2
HTLV 1
HTLV 2
LCMV
Mycoplasma sp.
Seoul
Sin Nombre

Negative

Pass

Procedures
  1. Remove human induced Pluripotent Stem Cells (hiPSC) from storage and maintain temperature until final thaw.
  2. Thaw hiPSC quickly in a 37°C water bath or mechanical thawing device. When using a water bath, swirl the sample gently until only a small ice crystal is visible. The approximate thaw time for a 1 ml sample in cryovial format is 2-3 min. The cryovial should be cool to the touch and SHOULD NOT warm to above chilled temperatures.
  3. Once thawed, the cell/cryomedia mix should be diluted into culture media (e.g. TeSR-E8™) at a ratio of 1:10 sample to media.
  4. Remove a sample for determination of cell viability and number.
  5. Plate hiPSC into an appropriate culture vessel (recommended density below).
  6. Once cells have adhered to the culture vessel, media should be exchanged with fresh media for downstream culture or differentiation.
  7. Atmosphere: 95% Air, 5% CO2
  8. Plate hiPSC at a density of 15,000 cells/cm2 in appropriate culture media.
  9. Change media daily and passage culture at 65-75% confluence using non-enzymatic dissociatio